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| 1. |
What is the human proteome map portal? |
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The
human proteome map portal is the web-based resource that
reorganizes mass spectrometry-based proteomics data to explore
expressed proteins in fetal tissues/adult tissues/hematopoietic
cells obtained from human. |
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| 2. |
Are these data from individuals with any known
diseases? |
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All samples used to generate these data were obtained from
histologically normal samples. |
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| 3. |
What is the quality of data? |
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All data were acquired on high-resolution Fourier transform
mass spectrometers using HCD method for fragmentation (high-high mode). |
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| 4. |
How are the expression values calculated? |
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Spectral counts per gene per experiment (e.g. SDS-PAGE) were first summed from all peptides mapped to each gene. Total acquired tandem mass spectra were used to normalize between experiments and then spectral counts per gene were averaged across multiple experiments (e.g. SDS-PAGE and bRPLC fractionation) per sample (e.g. pancreas). Final averaged spectral counts per gene per sample were used to plot the white-to-red gradient heat map for genes of interest, while summed spectral counts per peptide were used to depict the heat map for all peptides identified and mapped to a gene of interest. |
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| 5. |
How is the expression level displayed? |
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The protein expression of a gene is shown at the gene level on the basis of all peptides mapped to the given gene as a result of a query, and the abundance of peptides is displayed on the basis of the specificity at the gene level. |
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| 6. |
How do I download mass spectrometry based results
for the tissue of my interest? |
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The mass spectrometry proteomics data have been deposited to the ProteomeXchange Consortium via the PRIDE partner repository. The data can be accessed with PRIDE identifier PXD000561. |
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| 7. |
How can I cite the Human Proteome Map portal? |
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Kim et al. A draft map of the human proteome. 2014.
Nature.
509, 575-581. [PubMed] |
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